Journal: Stem Cells International

Article Title: Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists

doi: 10.1155/2019/7692973

Figure Lengend Snippet: Analysis of the effect of LPS preconditioning on the proregenerative ability of rBM-MSCs. (a–c) Photomontages showing the regenerated portions of the sciatic nerve that were inside the polyethylene tubes 4 weeks after transection. Axons were stained with TuJ1 antibodies (red). In the Vehicle group, only the Matrigel® solution was injected into the tube, while in the MSC and LPS-MSC groups, 5 × 10 5 rat bone marrow-derived mesenchymal stromal cells (rBM-MSCs) or lipopolysaccharide- (LPS-) preconditioned rBM-MSCs, respectively, were suspended in the Matrigel® solution and then injected into the tube immediately after sciatic nerve transection and tubulization. Scale bar: 200 μ m. (d–g) Quantitative analysis of nerve thickness, cell density, and number of TuJ1-positive axons (in the proximal and distal nerve segments) in each condition ( n = 5 rats per group; rBM-MSCs from only 1 animal were used in this experiment to reduce donor-related variability). Data are shown as individual values, median, and interquartile range. ∗ p < 0.05; Kruskal-Wallis test with Dunn's multiple comparisons test.

Article Snippet: 5 × 10 5 rBM-MSCs (or preconditioned rBM-MSCs), suspended in 15 μ L of a Matrigel® Growth Factor Reduced Basement Membrane Matrix (Corning) solution (1 : 3 in DMEM/F12 medium), were injected into the tube.

Techniques: Staining, Injection, Derivative Assay